Reader modes are customized to your specific applications with options to upgrade easily. No accessories are needed and SoftMax® Pro Software. The SpectraMax ® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The SpectraMax Plus 384 reader is equipped with the innovative technology patented by Molecular Devices, the PathCheck® Sensor which measures the depth of samples in a microplate through optical path length. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. Temperature control. In SOFTmax PRO, set up a plate section for an excitation scan with no cutoff filter and use the medium PMT setting. Microplate Automation & Detection. Example: [Drawer]. High sensitivity is demonstrated with a tryptophan standard curve, and a shift in peak emission is shown using a lysozyme denaturation assay. unconventional microplate reader assays, including imaging cytometry and western blots • Protocol sharing with a strong user community • Spectral optimization wizard to maximize assay signal window (SpectraMax i3x and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most published0200-6262. , a leading provider of high-performance life science solutions and a company of Danaher Corporation (NYSE:DHR), has launched its SpectraMax® Mini Multi-Mode Microplate. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. The 4 injector configuration enables flash and glow BRET assays and a 2-fold improvement in speed for traditional flash and glow assays. microplate reader. Compare plate readers The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. 5 87. Endpoint, kinetic, spectrum. 100 fmol 384-well (75 µL) 0. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). With optimized reagents, option to operate the. HTRF-compatible SpectraMax Multi-Mode Readers. The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with added functaionlity of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The system uses two scanning monochromators to determine the optimal excitation and emission settings. 2. Our SpectraTest® ABS2, FL1, LM1, SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86 The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. The SpectraDrop Microplate incorporates a specially designed adapter and a slide pair whose optical clarity allows measurements in absorbance and. For the GMP/GLP regulated labs, the systems come equipped with our SoftMax Pro GxP. Figure 1. These robust readers have been placed in labs from. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. . Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. Fill a clean plate with water. As a generous estimate, you might consider 300 W (at 120 Volts) per Spectramax reader, plus another 300 W for the Computer. 003 fmol 0. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. Transcreener ® ADP. The budget-friendly SpectraMax® Mini Multi-Mode Microplate Reader maximizes productivity without compromising sensitivity or performance. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. A SpectraMax M2 microplate reader helps minimize operating time and save costs, allowing researchers to spend more time analyzing data and generating practical knowledge. Fluorescence analysis of the colonies from the dilution series was performed in a microplate reader (SpectraMax iD3). SpectraDrop High-Throughput Screening (HTS) Kit. Our specialization servicing. This is equivalent to having 511 built-in filters,The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. Total volume (except deep. Due to elevated security of a Windows 7 64-bit operating system, the USB instrument drivers may not be installed automatically. Use similar settings for other Molecular Devices readers with fluorescence detection mode. Figure 1: Endotoxin standard curves on SpectraMax microplate readers, plotted using a log-log curve fit. The powerful analysis tools in the software generate a standard curve and calculated data. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. Preparation of Calibration Standards Calibration Standard (% Phosphorylated) µL Peptide Stock µL Phospho-peptide Stock 0 100 0 12. 306 plate air cal fail,low light. Contact our support team with your questions regarding Molecular Devices products and applications. . View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. The SpectraMax 340PC384 features correction for varying microplate well volumes and the elimination of standard curves based on PathCheck. The Molecular Devices Gemini XPS Fluorescent Microplate Reader provides an ideal environment to determine the optimal excitation and emission settings for most fluorescent assays. The SpectraMax® is a compact and economical solution designed to save researchers benchtop space and budget. If you do not have an iLab account you must first make one: Spectramax 190 absorbance microplate reader utilizes a patented multichannel optical design to simulate a dual beam spectrophotometer to help eliminate errors through the usage of a dual fiber optic exposure. 315 can't find zero order. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. Microplate Readers Molecular Devices SpectraMax M2E Microplate Reader. The chamber contains springs that position the cuvette in the proper alignment for a read. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. The following document is intended to demonstrate setup of this instrument. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. When combined with the SpectraDrop Micro-Volume Microplate, the reader can quantitate up to 64 samples with as little as 2 µL sample volume in a. Example: [Drawer]. on the SpectraMax iD3 microplate reader Introduction The intrinsic fluorescence of proteins is due to the aromatic amino acids tryptophan, tyrosine, and phenylalanine. Figure 1. 0 out of 5 $ 23,995 $ 22,495. We use Spectramax M5 microplate reader in our lab. 5 12. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. SpectraMax Microplate Readers. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command. Figure 1. 7°C and the bottom to 36. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. M2. Additionally, user-installable options allow the SpectraMax i3 System to. their consistent performance, reliability and durability. Thank you!. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. unconventional microplate reader assays, including imaging cytometry and western blots • Protocol sharing with a strong user community • Spectral optimization wizard to maximize assay signal window (SpectraMax i3 and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most publishedi3x. B: Selection of Excitation Wavelength. The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 850 nm). e. Cuvette Microplate wells. microplates or in a cuvette at a. 305 reference level saturation. SpectraMax ® M2 Multi-Mode Microplate Reader. Lower limits of detection for ATP determined using the ATPliteAbsorbance was read at 590 nm on the SpectraMax ABS Plus Microplate Reader. Selection of Water Constant in PathCheck settings. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). The Quant-iT PicoGreen dsDNA Assay Kit, when run on a SpectraMax microplate reader with SoftMax Pro Software, is a quick, sensitive detection method for double-stranded DNA. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. Molecular Devices SpectraMax M2 Multimode Plate Reader. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 3) This warranty. Optional enhancements • SpectraMax MiniMax Imaging Cytometer • ScanLater Western Blot System • SpectraDrop™ Micro-Volume Microplate 312 gain calibration failed. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. In addition, the SpectraMax iD5Next generation SpectraMax® absorbance microplate readers. Other technologies such as imaging. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. The calculated LOQ values were 0. Page 35: Setting Up The Instrument SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . IMAP FP Settings in SoftMax Pro SpectraMax M5 Multi-Mode Microplate Reader. SpectraMax L Microplate Reader This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Or scan up to 384 wells at any time to determine the best assay conditions. Gemini XPS Microplate Reader (or other SpectraMax reader with fluorescence detection mode) SoftMax Pro Software; Methods Set up the instrument and software. 0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingSpectraMax i3x. The drawing shows a standard microplate of 15 mm height covered with a lid. Users can install the ScanLater Western Blot Detection Cartridge in the SpectraMax Paradigm SystemMicroplate readers read biological, chemical or physical events that occur on a microtiter plate. SpectraMax Plus: SpectraMax Plus. Examples: “Press carefully to engage,” “ Do not press down. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. On the back, in the right hand top corner,. The eight-channel system- consisting of 8 sample beams and 8 reference beams delivers both superior. Devices’ StakMax® Microplate Stacker in a matter of minutes and . SpectraMax iD5 Multi-Mode Microplate Reader Optional, for SpectraMax iD5 reader: Fluorescence Filter with holder 565nm BW 30nm (Molecular Devices cat. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerThe SpectraDrop microplate was read on the SpectraMax ® Paradigm ® Multi-Mode Microplate Reader using the Fluorescence Intensity detection cartridge (FI-FLRH) with fluorescein filters, or Tunable Wavelength Selection (TUNE) cartridge with signal/ background-optimized wavelengths. The dual monochromator optics allow the widest range of. . This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. Figure 2-23: Bottle holder To install the bottle holder: 1. Each sample has a discrete sample beam and reference beam so that each well is measured directly. More Product Information. The SpectraMax® ABS Plus Microplate Reader can run . 030 fmol Fluorescence Polarization (FP). Find regional support. Introduction. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. 7°C and the bottom to 36. The SpectraMax ® iD5 Multi-Mode Microplate Reader includes an absorbance detection mode optimal for protein quantitation assays. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. com 4. Introduction. Summary of Contents for Molecular Devices SpectraMax M2. These lab workhorses are used in drug discovery. Choose from a three- (M3), four- (M4), orIn this case, the SpectraMax i3x reader works as a flash-lamp based microplate reader as described in the supporting application note “The AutoPMT™ feature for an extended dynamic range in the SpectraMax iD and M series Multi-Mode Readers”. Narrow bandwidths deliver increased measurement accuracy and linearity over the widest range of assays, including. 0 out of 5 $ 11,999. Find regional support. No additional manuals are available. Microplate Readers Molecular Devices Spectramax M5 Microplate Reader. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. We would like to show you a description here but the site won’t allow us. flat meniscus), but is approx 15 mL. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. SPECTRAmax PLUS Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. ; Page 2 2) All labor charges to repair the product for a period of one (1) year from the original date of delivery will be paid by Molecular Devices Corporation. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile instrument that can measure absorbance, fluorescence and luminescence in microplates. limit of detection (LLD) for ATP was estimated for each microplate reader using the . The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. However, this method is only able to measure down to about 250 ng/mL on a typical absorbance microplate reader. 6 for further experiment, so I'm confused which i would choose for OD value determination. Click SoftMax Pro Software User Guide. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Table 1. The system uses two scanning monochromators to determine the optimal excitation and emission settings. Large, high resolution touchscreen interface allows you to easily set up methods, run experiments or view tutorial videos without the need for a dedicated computer workstation. Absorbance (optical density, OD) was read at 570 nm and 605. Add to Cart. Download PDF. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. Plate chamber design of the SpectraMax category 1 microplate readers. SpectraMax Glo Steady-Luc Reporter Assay Kit workflow. Brochure. Three low volume 24-well bottom slides. SpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. ELISA Plate Readers / Microplate Readers. For the calculation of the ratiometric biosensor signal, the emission intensity at 510 nm was recorded upon an excitation at 380 nm and 470 nm and the former was divided by the latter (Exc. The advanced optical and. Supplier: Molecular Devices. For readers without a cuvette port, simply check the box next to ‘PathCheck’. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. Here, we illustrate how the SpectraMax iD5 reader, in combination with SoftMax ® Pro Software, is used to quantitate a cellular protein sample with two different kits, Pierce BCA Protein Assay Kit and Pierce Rapid. The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. FI assay. All . The SpectraMax ® M5e Multi-Mode Microplate Reader is a dualmonochromator capable of fluorescence, time-resolved fluorescence (TRF), fluorescence polarization, absorbance, and luminescence assays. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Use the provided 3. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. \ Figure 3. keeping with best practices, reader performance should. The SpectraMax M2 and M2e are versatile and reliable instruments that can perform a range of applications in. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. Published Dec 16, 2020 | Updated May 19, 2023. (A) AccuBlue Pico kit; (B) AccuClear Nano kit; (C. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. , April 14, 2022 – Molecular Devices, LLC. Examples: “Press carefully to engage,” “ Do not. With three modes of detection for UV/Vis absorbance, fluorescence and luminescence, the SpectraMax® Mini reader is user-upgradeable - from two to three modes, providing researchers with a versatile instrument. The Molecular Devices Gemini XS Fluorescent Microplate Reader lets you experiment with a variety of fluorophores and can even find the best excitation and emission wavelengths for new assays. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. Step 3: Use the Template Editor in the cell standards section of the protocol to create a template of the assay indicating the location of standards (cell numbers for each dilution. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. 2. SpectraMax® Microplate Reader Suite of Products Knowledge Base Published Dec 16, 2020 | Updated Dec 06, 2021 Products : SpectraMax i3x reader,. Dual-Glo luciferase assay system (Promega Cat. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. View the Molecular Devices website. The price range of a reader starts at 2. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). Microplate Readers BioTek Epoch 2 Microplate Reader. Fluorescence Microplate Reader Comparison Chart. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. Nucleic Acid and Protein Quantitation. This variable pathlength makes it dif Þcult to perform extinction-based assays and confusing to compare results between microplate readers and spectrophotometers. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial. following formula: LLD = 3*SD blank *Concentration/(RLU sample-RLU blank). . 33 Dramatic aggregation of Alzheimer A b by Cu(II) is induced by conditions representing physiological acidosis . microplate reader. Unlock your i3/i3x instrument after shipment. Download the SpectraMax Mini Reader Plug-in and associated guides. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. The SpectraMax iD3 reader is fully compatible with the SpectraDropTM Micro Volume Microplate, enabling quantitation of precious low volume samples. Keywords: spectramax mini multi-mode microplate reader, spectramax mini, spectramax mini microplate reader Created Date: 5/25/2022 9:57:03 AMFully Warranty. Molecular Devices. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. both cuvette-based and microplate reader applications on the same instrument. Microplate readers use a vertical light path so the distance of the light through the sample depends on the volume. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. The plate reader optics are tunable for. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. The SpectraMax Multi-Mode Microplate Readers are packed in a specially designed carton. The optimized settings listed in Table 1 can be transferred to any plate reader of the same model, i. This application note describes experimental protocols that enable the detection of relatively low numbers of cells expressing Green Fluorescent Protein. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. Introduction. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. Learn about the features, specifications and applications of SpectraMax M2/M2e Microplate Readers, a multi-detection system with dual-mode cuvette ports and dual monochromators. For readers without a cuvette port, simply check the box next to ‘PathCheck’. Add to Cart. SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 9 PathCheck ® Pathlength Meas urement Technology A SpectraMax Multi-Mode Microplate Reader with PathCheck Pathlength Measurement Technology allows normalization of variable well volumes to 1-cm cuvette readings. SpectraMax Quant dsDNA Assay Kits are highly selective for dsDNA. Spectranet customer portalthe SpectraMax® M Series Multi-Mode Microplate Readers, for . 100 readings per well gives optimal Z’ values for this assay. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The SpectraMax® iD3 and iD5 measure absorbance, fluorescence, and luminescence. An assay plate containing a 15-point standard curve was read on the SpectraMax. Using the. SpectraMax Spectrofluorometers. SpectraMax Microplate Readers. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. begin reading microplates with seven mouse clicks. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. The plate reader optics are tunable for absorbance. 76358-624. 316 grating motor driver faulty. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. IMAP FP KINASE ASSAYS ON THE SPECTRAMAX M5 MULTI-MODE MICROPLATE READER 校准标准品的制备 Table 1. 6% for the on-board optics (monochromators). 0 out of 5The SpectraMax ® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. Sale. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. Reporter Assay. The instrument uses a paired monochromator and xenon flash. 0200-6263. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. The SpectraMax L reader is available in 1- and 2-channel (Photomultiplier Tube) configurations. The Molecular Devices SpectraMax M2e Microplate Reader makes UV-Vis absorbance (200-1000 nm), fluorescence intensity (excitation 250-850 nm, emission 250-850 nm), chemiluminescence intensity (250-850 nm), and. Assays vary depending on temperature or pH and luminescence microplate reader validation DATA SHEET. To resume collecting kinetic measurements within the same file, press the Append button. The SpectraMax® M3 Multi-Mode Microplate Reader is a UV/visible reader with dual monochromatic optics and three modes of detection for bioresearch procedures. The 4 injector configuration enables flash and glow BRET assays and a 2-fold improvement in speed for traditional flash and glow assays. It is user upgradeable and budget friendly, and comes with a brochure that provides detailed information on its features, specifications and applications. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. no. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. If it looks fine, then proceed. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. SpectraMax 250 User. We would like to show you a description here but the site won’t allow us. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). 72 MB. View your data quickly using the large touchscreen interface, export your data for analysis in the program of. Our absorbance plate readers feature our PathCheck Sensor technology and. The Molecular Devices SpectraMax XPS has an optical design that is the only multi-channel system that truly mimics a dual-beam spectrophotometer. Microplate Readers Thermo Fluoroskan Ascent FL Fluorescence Microplate Reader. keeping with best practices, reader performance should still be validated and documented regularly, especially in regulated laboratories. The SpectraMax® M3 Multi-Mode Microplate Reader is a UV/visible reader with dual monochromatic optics and three modes of detection for bioresearch procedures. Find our technical support information along with contact details. MPN:SpectraMax Plus. Put 200 µL sample into one or more wells of a microplate and 200 µL buffer or solvent into one or more other wells. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile instrument that. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. The SpectraMax ABS Plus Microplate Reader is a compact and adaptive microplate reader capable of quantitating nucleic acid and protein samples in various formats such as cuvettes or microplates. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. Download PDF. The instruments affected are the SpectraMax ® L Microplate Reader and the StakMax® Microplate Handling System. Data exemplifying linear ranges from the three different kits are presented in Figure 2. The fluorometric performance of the SpectraMax M2e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. Minimize manual manipulation of samples and data processing, and improve laboratory efficiency. The temperature in the plate chamber is ambient and isothermal. Results are shown in Table 1. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with streamlined workflows to ensure data integrity. 1. Published Dec 16, 2020 | Updated Dec 16, 2020. Step 1: Open the SoftMax Pro IMAP FP protocol. Eight differentSpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. It offers the additional advantage of HTRF ® (homogeneous time-resolved fluorescence) certification, allowing. Sensitive fluorescent quantitation of DNA with the Quant-iT. 314 low lamp level warning. With optimized reagents, option to operate the. It features a modular design that allows users to add or change detection cartridges according to their needs. The drawing shows a standard microplate of 15 mm height covered with a lid. Music: Cylinder Six by Chris Zabriskie is licensed under a Creative Commons Attribution license (microplate reader is a device used to detect chemical, biological, or physical reactions by measuring emitted light. Microplate automation and detection instruments for walk away operation and high quality results. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Also see:. In addition, the iD5 reader measures TRF and FP. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application. Improve your lab’s efficiency, get more data, and decrease time to discovery with an automated microplate washer and reader solution: The SpectraMax® Mini Multi-Mode. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. For a complete list of the instruments supported by this release of the SoftMax Pro Software, see Supported Instruments on page 111. Software validation, IQ/OQ/PQ and FDA 21 CFR Part 11 compliance tools are also available. The narrow bandwidths used by the model increase measurement accuracy and linearity over a wide. SpectraMax® ABS Plus readers use a grating monochromator to select the exact wavelength needed for every assay. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. SpectraMax iD3 Multi-Mode Microplate Reader Unparalleled performance on a personalized platform The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks.